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Addgene

MLM3639
(Plasmid #42252)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 42252 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    unknown
  • Vector type
    CRISPR ; zebrafish expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Streptococcus pyogenes Cas9-3X Flag
  • Alt name
    Cas9-002
  • Promoter CMV
  • Tag / Fusion Protein
    • 3X Flag (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid Features:
CAG enhancer-CMV promoter = 7466-370
Cas9 = 409-4512
NLS = 4519-4539
3X FLAG = 4546-4611
BGHpA = 4612-4839

For more information on Joung Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/jounglab/

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MLM3639 was a gift from Keith Joung (Addgene plasmid # 42252 ; http://n2t.net/addgene:42252 ; RRID:Addgene_42252)

  • For your References section:

    Efficient genome editing in zebrafish using a CRISPR-Cas system. Hwang WY, Fu Y, Reyon D, Maeder ML, Tsai SQ, Sander JD, Peterson RT, Yeh JR, Joung JK. Nat Biotechnol. 2013 Jan 29. doi: 10.1038/nbt.2501. 10.1038/nbt.2501 PubMed 23360964
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