pCMV5-rat ERK2-L73P/S151D
(Plasmid #40819)

• Depositing Lab: Natalie Ahn
• Publication: Emrick et al J Biol Chem. 2001 Dec 7;276(49):46469-79. Epub 2001 Oct 8.

Backbone

• Vector backbone: pCMV5
• Backbone manufacturer: David Russell, UTSW
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 6200
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: MAPK1
• Alt name: ERK2
• Alt name: p42
• Species: R. norvegicus (rat)
• Insert Size (bp): 1500
• Mutation: L73P, S151D
• GenBank ID: NM_053842
• Entrez Gene: Mapk1 (a.k.a. ERK-2, ERT1, Erk2, p42-MAPK)
• Promoter: CMV
• Tag / Fusion Protein:
  • His6 (N terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Melanie Cobb, UTSW
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry