pET-His6-ERK2-MEK1_R4F_coexpression
(Plasmid #39212)

• Depositing Lab: Melanie Cobb
• Publication: Khokhlatchev et al J Biol Chem. 1997 Apr 25;272(17):11057-62.

Backbone

• Vector backbone: pET
• Backbone size w/o insert (bp): 4600
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: Mek1 R4F
• Species: H. sapiens (human)
• Mutation: MEK1(R4F) = S218E, S222D and deletion of AA32-51
• Entrez Gene: MAP2K1 (a.k.a. CFC3, MAPKK1, MEK1, MEL, MKK1, PRKMK1)
• Promoter: T7
• Tag / Fusion Protein:
  • RBS (C terminal on backbone)

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: NA (unknown if destroyed)
• 3′ cloning site: NA (unknown if destroyed)
• 5′ sequencing primer: T7

Gene/Insert 2

• Gene/Insert name: Erk2
• Species: R. norvegicus (rat)
• Entrez Gene: Mapk1 (a.k.a. ERK-2, ERT1, Erk2, p42-MAPK)
• Promoter: T7 via RBS
• Tag / Fusion Protein:
  • His6 (N terminal on insert)

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: NA (unknown if destroyed)
• 3′ cloning site: NA (unknown if destroyed)
• 5′ sequencing primer: none
• 3′ sequencing primer: rErk2-R

Resource Information

• Articles Citing this Plasmid:
  • 7 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry