Skip to main content
Addgene

NpT7-5-Erk2
(Plasmid #39230)

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 39230 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    NpT7-5
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Erk2
  • Species
    R. norvegicus (rat)
  • GenBank ID
    M64300
  • Entrez Gene
    Mapk1 (a.k.a. ERK-2, ERT1, Erk2, p42-MAPK)
  • Promoter T7
  • Tag / Fusion Protein
    • His (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    NpT7-5-Erk2 was a gift from Melanie Cobb (Addgene plasmid # 39230 ; http://n2t.net/addgene:39230 ; RRID:Addgene_39230)
  • For your References section:

    Regulation and properties of extracellular signal-regulated protein kinases 1 and 2 in vitro. Robbins DJ, Zhen E, Owaki H, Vanderbilt CA, Ebert D, Geppert TD, Cobb MH. J Biol Chem. 1993 Mar 5;268(7):5097-106. PubMed 8444886