pET21a-MKP7_K55R_153
(Plasmid #37794)

• Depositing Lab: SeWon Suh
• Publication: Kim et al Proc Natl Acad Sci U S A. 2012 May 15;109(20):7729-34. Epub 2012 Apr 30.

Backbone

• Vector backbone: pET21a
• Backbone size w/o insert (bp): 5500
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Mitogen-activated protein kinase phosphatase 7
• Alt name: MKP7
• Alt name: DUSP16
• Species: H. sapiens (human)
• Insert Size (bp): 459
• Mutation: changed Lysnie 55 to Arginine and deleted amino acids 154-665
• Entrez Gene: DUSP16 (a.k.a. MKP-7, MKP7)
• Tag / Fusion Protein:
  • His (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: T7 terminator

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry