pCFJ594 - pENTR[1-2](NeoR)
(Plasmid #34878)

• Depositing Lab: Erik Jorgensen
• Publication: Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi: 10.1038/nmeth.1865.

Backbone

• Vector backbone: pDONRP2-P3R
• Vector type: Worm targeting
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NeoR
• Promoter: rps-27

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13F
• 3′ sequencing primer: M13R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: We would like to thank Rosina Giordano-Santini and Denis Dupuy for sending us the original Neomycin Resistance plasmid. For details on how to use the neomycin selection in C. elegans and other nematode species please see: Giordano-Santini R, Milstein S, Svrzikapa N, Tu D, Johnsen R, Baillie D, Vidal M, Dupuy D. Nat Methods. 2010 Sep;7(9):721-3. An antibiotic selection marker for nematode transgenesis.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry