LAMP1-mGFP
(Plasmid #34831)

• Depositing Lab: Esteban Dell'Angelica
• Publication: Falcon-Perez et al J Cell Sci. 2005 Nov 15;118(Pt 22):5243-55. Epub 2005 Oct 25.

Backbone

• Vector backbone: pEGFP-N3
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4729
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: LAMP1
• Species: H. sapiens (human)
• Insert Size (bp): 1300
• Entrez Gene: LAMP1 (a.k.a. CD107a, LAMPA, LGP120)
• Promoter: CMV
• Tags / Fusion Proteins:
  • mGFP (C terminal on backbone)
  • Kozak (N terminal on insert)
  • GSTGSTGSTGA linker (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: CMV-Fwd
• 3′ sequencing primer: EGFP-N

Resource Information

• Articles Citing this Plasmid:
  • 79 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid contains a variant of GFP, where the leucine at position 221 has been replaced by lysine to avoid anti-parallel dimerization.