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Addgene

pGFP(C3)-Vinculin
(Plasmid #30312)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 30312 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGFP-C3
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Vinculin
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    3200
  • Mutation
    F633S, V828E, L936P in mouse viniculin (NM_009502.4)
  • Entrez Gene
    Vcl (a.k.a. 9430097D22)
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRi (not destroyed)
  • 3′ cloning site EcoRi (not destroyed)
  • 5′ sequencing primer GFP-3'-Fw
  • 3′ sequencing primer pGFP-Re
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGFP(C3)-Vinculin was a gift from Klaus Hahn (Addgene plasmid # 30312 ; http://n2t.net/addgene:30312 ; RRID:Addgene_30312)
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