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Addgene

pENTR5'_ubi
(Plasmid #27320)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 27320 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pENTR5' TOPO
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2692
  • Vector type
    Multisite Gateway 5' entry vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    zebrafish ubiquitin promoter
  • Alt name
    ubi
  • Alt name
    pCM206
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    3483
  • Entrez Gene
    ubb (a.k.a. Ubi-p63E, im:6892314, si:ch211-202a12.3, ubc, zUBC, zgc:172187)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site attL4 / XhoI (not destroyed)
  • 3′ cloning site attR1 / BamHI (not destroyed)
  • 5′ sequencing primer M13 forward
  • 3′ sequencing primer M13 reverse
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pENTR5'_ubi was a gift from Leonard Zon (Addgene plasmid # 27320 ; http://n2t.net/addgene:27320 ; RRID:Addgene_27320)

  • For your References section:

    Ubiquitous transgene expression and Cre-based recombination driven by the ubiquitin promoter in zebrafish. Mosimann C, Kaufman CK, Li P, Pugach EK, Tamplin OJ, Zon LI. Development. 2011 Jan . 138(1):169-77. 10.1242/dev.059345 PubMed 21138979
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