pGEX-αTAT1[2-236]
(Plasmid #27101)

• Depositing Lab: Maxence Nachury
• Publication: Shida et al Proc Natl Acad Sci U S A. 2010 Nov 10. ():.

Backbone

• Vector backbone: pGEX-6P1
• Backbone size w/o insert (bp): 4981
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: αTubulin K40 acetyltransferase
• Alt name: αTAT1
• Species: H. sapiens (human)
• Insert Size (bp): 708
• Entrez Gene: ATAT1 (a.k.a. C6orf134, MEC17, Nbla00487, TAT, alpha-TAT, alpha-TAT1)
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamH1 (not destroyed)
• 3′ cloning site: EcoR1 (not destroyed)
• 5′ sequencing primer: pGEX-Foward
• 3′ sequencing primer: pGEX-Reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry