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Addgene

pmCherry-CIBN-CreC
(Plasmid #26889)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26889 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMCherryC1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4722
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CIBN-CreC
  • Alt name
    truncated CIB1, Cre recombinase C terminal fragment
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    1274
  • Mutation
    aa 1-170 of CIB1, aa 106-343 of Cre
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Sac I (not destroyed)
  • 3′ cloning site Xma I (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pmCherry-CIBN-CreC was a gift from Chandra Tucker (Addgene plasmid # 26889 ; http://n2t.net/addgene:26889 ; RRID:Addgene_26889)

  • For your References section:

    Rapid blue-light-mediated induction of protein interactions in living cells. Kennedy MJ, Hughes RM, Peteya LA, Schwartz JW, Ehlers MD, Tucker CL. Nat Methods. 2010 Dec;7(12):973-5. doi: 10.1038/nmeth.1524 10.1038/nmeth.1524 PubMed 21037589
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