pmCherry-CRY2-CreN
(Plasmid #26888)

• Purpose: Expresses N-terminal fragment of Cre fused to CRY2 for blue light-induced activation of DNA recombination when used with CIBN-CreC. Also expresses mCherry.
• Depositing Lab: Chandra Tucker
• Publication: Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi: 10.1038/nmeth.1524

Backbone

• Vector backbone: pMCherryC1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4722
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CRY2-CreN
• Alt name: Cryptochrome 2, Cre recombinase N terminal fragment
• Species: A. thaliana (mustard weed)
• Insert Size (bp): 2151
• Mutation: aa 19-104 of Cre
• Entrez Gene: cre (a.k.a. P1_gp003)
• Entrez Gene: CRY2 (a.k.a. AT1G04400, AT-PHH1, ATCRY2, CRYPTOCHROME 2 APOPROTEIN, F19P19.14, F19P19_14, FHA, PHH1, cryptochrome 2)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Sac I (not destroyed)
• 3′ cloning site: Xma I (not destroyed)
• 5′ sequencing primer: n/a

Resource Information

• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry