pSLIK-Zeo
(Plasmid #25736)

• Purpose: (Empty Backbone) Lentiviral vector for Tet-based inducible shRNA or cDNA expression, constitutive Zeocin resistance gene coexpression.
• Depositing Lab: Iain Fraser
• Publication: Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 103(37):13759-64.

Backbone

• Vector backbone: pSLIK
• Backbone manufacturer: Iain Fraser
• Backbone size (bp): 12944
• Vector type: Mammalian Expression, Lentiviral, RNAi
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DB3.1
• Growth instructions: DB3.1 (ccdB survival)
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: None

Cloning Information

• Cloning method: Gateway Cloning
• 5′ cloning site: attR1 (not destroyed)
• 3′ cloning site: attR2 (not destroyed)
• 5′ sequencing primer: CMV-F, hUBCpro-F
• 3′ sequencing primer: hUBCpro-R

Resource Information

• Addgene Notes:
  • Addgene diagnostic digest
• A portion of this plasmid was derived from a plasmid made by: rtTA3- Atze Das, University of Amsterdam; ZeoR- Greg Hannon, CSHL; pDS cassette- Invitrogen
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Parent lentivector, co-expression of Zeocin selection gene