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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 25648 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV (Strategene)
- Backbone size w/o insert (bp) 5020
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name0
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Age I, Asc I, Bcl I, Mlu I, Nhe I, Sac I, Xba I, Spe I (not destroyed)
- 3′ cloning site NdeI, EcoR I, EcoR V, Cla I, Sal I, Xho I (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that an 11bp deletion was found in the 3' ITR ("AAV2 alternate). The depositing lab was not able to confirm whether this was present in their original stock.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-SEPT-Acceptor was a gift from Todd Waldman (Addgene plasmid # 25648 ; http://n2t.net/addgene:25648 ; RRID:Addgene_25648) -
For your References section:
Epitope tagging of endogenous genes in diverse human cell lines. Kim JS, Bonifant C, Bunz F, Lane WS, Waldman T. Nucleic Acids Res. 2008 Nov . 36(19):e127. 10.1093/nar/gkn566 PubMed 18784188