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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 24956 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGL2
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Vector typeMammalian Expression, Luciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBNP promoter
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SpeciesH. sapiens (human)
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Entrez GeneNPPB (a.k.a. BNP, Iso-ANP)
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Tag
/ Fusion Protein
- Luciferase (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer N/A
- 3′ sequencing primer LucNRev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
An authentic NFAT3 reporter was constructed containing two tandem copies of the sequence 5′-CGCGTCTATCCTTTTGTTTTCCATCCTG-3′ derived from the BNP promoter and previously demonstrated to bind NFAT3.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBNP-NFAT-luciferase was a gift from Toren Finkel (Addgene plasmid # 24956 ; http://n2t.net/addgene:24956 ; RRID:Addgene_24956)
