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Addgene

pRR-MR-5Z
(Plasmid #23059)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 23059 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRW95-3
  • Backbone manufacturer
    PMID: 8800671
  • Backbone size w/o insert (bp) 8700
  • Vector type
    Yeast Expression
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MR
  • Alt name
    mineralocorticoid receptor
  • Species
    H. sapiens (human)
  • Mutation
    N528H, Q814R, H993L
  • GenBank ID
    AAA59571.1
  • Entrez Gene
    NR3C2 (a.k.a. MCR, MLR, MR, NR3C2VIT)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SnaBI (destroyed during cloning)
  • 3′ cloning site SnaBI (destroyed during cloning)
  • 5′ sequencing primer GAL1
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The cDNA used to create this plasmid was obtained from Ron Evans.
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRR-MR-5Z was a gift from Charles Miller (Addgene plasmid # 23059 ; http://n2t.net/addgene:23059 ; RRID:Addgene_23059)

  • For your References section:

    Single plasmids expressing human steroid hormone receptors and a reporter gene for use in yeast signaling assays. Miller CA, Tan X, Wilson M, Bhattacharyya S, Ludwig S. Plasmid. 2009 Dec 3. ():. 10.1016/j.plasmid.2009.11.003 PubMed 19962400
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