pLH121
(Plasmid #22696)

• Depositing Lab: Lynn Hudson
• Publication: Nadon et al J Neurochem. 1994 Sep . 63(3):822-33.

Backbone

• Vector backbone: pTZ19R
• Backbone manufacturer: Fermentas
• Backbone size w/o insert (bp): 2862
• Vector type: Phagemid

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: DH5 alpha (normal growth conditiions)
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Human Proteolipid Protein cDNA
• Alt name: PLP
• Species: H. sapiens (human)
• Insert Size (bp): 9000
• Entrez Gene: PLP1 (a.k.a. GPM6C, HLD1, MMPL, PLP, PLP/DM20, PMD, SPG2)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: M13/pUC (-20)
• 3′ sequencing primer: M13/pUC reverse

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The human PLP genomic and cDNA was cloned in Hudson lab

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This was the PLP transgene construct: consists of the human PLP cDNA, flanked on the 5' side by 4.2 kb of PLP promoter sequence from the human genomic DNA and on the 3' side by 1.5 kb of genomic DNA from the 3' end of the human PLP gene.