pPLP1.5
(Plasmid
#22614)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 22614 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepUC8
- Backbone size w/o insert (bp) 2665
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Vector typeE. Coli phagemid vector
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsDH5 alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namehuman Proteolipid Protein cDNA
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SpeciesH. sapiens (human)
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Insert Size (bp)1500
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GenBank IDAK295388
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Entrez GenePLP1 (a.k.a. GPM6C, HLD1, MMPL, PLP, PLP/DM20, PMD, SPG2)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer n/a
- 3′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The 1500bp Human Proteolipid protein (PLP) was subcloned from a lambda gt11 human brainstem cDNA library
This PLP DNA cloned by Hudson lab.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPLP1.5 was a gift from Lynn Hudson (Addgene plasmid # 22614 ; http://n2t.net/addgene:22614 ; RRID:Addgene_22614) -
For your References section:
Myelin-specific proteolipid protein is expressed in myelinating Schwann cells but is not incorporated into myelin sheaths. Puckett C, Hudson L, Ono K, Friedrich V, Benecke J, Dubois-Dalcq M, Lazzarini RA. J Neurosci Res. 1987 . 18(4):511-8. 10.1002/jnr.490180402 PubMed 2449540