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Addgene

FRB-mCherry-EEA1
(Plasmid #222419)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 222419 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    FRB-mCherry-C1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    EEA1
  • Species
    H. sapiens (human)
  • Entrez Gene
    EEA1 (a.k.a. MST105, MSTP105, ZFYVE2)
  • Tag / Fusion Protein
    • FRB-mCherry (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.05.31.596797 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FRB-mCherry-EEA1 was a gift from Stephen Royle (Addgene plasmid # 222419 ; http://n2t.net/addgene:222419 ; RRID:Addgene_222419)

  • For your References section:

    Non-disruptive inducible labeling of ER-membrane contact sites using the Lamin B Receptor. Downie L, Ferrandiz N, Megan Jones M, Royle SJ. bioRxiv 2024.05.31.596797 10.1101/2024.05.31.596797
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