pHAPE
(Plasmid
#217451)
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Purpose(Empty Backbone) For digestion with restriction enzymes to create DNA size marker ladders
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 217451 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBluescript II SK +
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Backbone manufacturerStratagene
- Backbone size (bp) 2859
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Modifications to backbone2x pairs of BamHI and HindIII restriction sites introduced
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Vector typeDNA size markers
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHAPE was a gift from Michael Lardelli (Addgene plasmid # 217451 ; http://n2t.net/addgene:217451 ; RRID:Addgene_217451) -
For your References section:
pHAPE: a plasmid for production of DNA size marker ladders for gel electrophoresis. Allen AG, Barthelson K, Lardelli M. Biol Methods Protoc. 2023 Jul 31;8(1):bpad015. doi: 10.1093/biomethods/bpad015. eCollection 2023. 10.1093/biomethods/bpad015 PubMed 37547169