-
Depositing Lab
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 21741 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepEYFP-N1
-
Backbone manufacturerClonetech
- Backbone size w/o insert (bp) 4733
-
Vector typeMammalian Expression
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameClathrin, light polypeptide (LCa)
-
Alt nameClta
-
SpeciesM. musculus (mouse)
-
Insert Size (bp)657
-
GenBank IDNM_001080385
-
Entrez GeneClta (a.k.a. Lca)
-
Tag
/ Fusion Protein
- EYFP (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Kpn1 (not destroyed)
- 3′ cloning site Age1 (not destroyed)
- 5′ sequencing primer EGFP-N, Clonetech# 6479-1
- 3′ sequencing primer EGFP-C, Clonetech# 6478-1 (Common Sequencing Primers)
Resource Information
-
A portion of this plasmid was derived from a plasmid made byGift of J.H. Keen, Nat. Cell Biol. 1,1,(1999)
-
Articles Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
Clathrin-LCa-EYFP was a gift from Xiaowei Zhuang (Addgene plasmid # 21741 ; http://n2t.net/addgene:21741 ; RRID:Addgene_21741) -
For your References section:
Epsin 1 is a cargo-specific adaptor for the clathrin-mediated endocytosis of the influenza virus. Chen C, Zhuang X. Proc Natl Acad Sci U S A. 2008 Aug 19. 105(33):11790-5. 10.1073/pnas.0803711105 PubMed 18689690