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pLV6-mPER2-luc
(Plasmid #212035)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 212035 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLenti6
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 6339
  • Total vector size (bp) 8540
  • Modifications to backbone
    A additional BamHI restricition site was added by site-directed mutagenesis 5' of the insert
  • Vector type
    Mammalian Expression, Lentiviral, Luciferase
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mPer2-luciferase
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2201
  • Entrez Gene
    Per2 (a.k.a. mKIAA0347, mPer2)
  • Tag / Fusion Protein
    • luciferase (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer M13
  • 3′ sequencing primer M13
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Steven Brown (Addgene plasmid # 68833) and Joseph Takahashi (Addgene plasmid # 48747 ).

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The insert (mPer-luciferase) derived from the pGL3 basic E2 vector (Plasmid #48747, a gift from Joseph Takahashi). The pLV6 backbone derived from the pLV6-Bmal-luc vector (Plasmid #68833, a gift from Steven Brown). The pLV6-Bmal-luc vector contains one BamHI cutting site 3’ of the Bmal1-luciferase insert. Site-directed mutagenesis was used to create an additional BamHI cutting site 5’ of the insert. The mPer2-luc insert of the pGL3-Per2-luc vector is by default flanked by BamHI cutting sites. After successful digestions and extractions, the pLV6 backbone and the mPer2-luc fragment were ligated with T4 DNA ligase, hence obtaining the pLV6-mPer2-luc vector.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV6-mPER2-luc was a gift from David Hazlerigg (Addgene plasmid # 212035 ; http://n2t.net/addgene:212035 ; RRID:Addgene_212035)

  • For your References section:

    The Reindeer Circadian Clock Is Rhythmic and Temperature-compensated But Shows Evidence of Weak Coupling Between the Secondary and Core Molecular Clock Loops. Appenroth D, Ravuri CS, Torppa SK, Wood SH, Hazlerigg DG, West AC. J Biol Rhythms. 2024 Dec;39(6):554-567. doi: 10.1177/07487304241283066. Epub 2024 Oct 6. 10.1177/07487304241283066 PubMed 39370744
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