PB-TET-mKLF4
(Plasmid #20906)

• Purpose: piggyBac vector with tet inducible mKLF4
• Depositing Lab: Andras Nagy
• Publication: Woltjen et al Nature. 2009 Mar 1. ():.

Backbone

• Vector backbone: PB-TET
• Backbone size w/o insert (bp): 9186
• Vector type: Mammalian Expression ; piggyBac
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: KLF4
• Species: M. musculus (mouse)
• Insert Size (bp): 1479
• Entrez Gene: Klf4 (a.k.a. EZF, Gklf, Zie)
• Tag / Fusion Protein:
  • IRES-bGeo (C terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ cloning site: attB1 (not destroyed)
• 3′ cloning site: attB2 (not destroyed)
• 5′ sequencing primer: M13 F, T7
• 3′ sequencing primer: M13 R, T3

Resource Information

• A portion of this plasmid was derived from a plasmid made by: KLF4 gateway cloned from Yamanaka Plasmid 13370: pMXs-Klf4, piggyBac terminal repeats obtained from Pentao Liu at Sanger.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • piggyBac Limited Use Label License
• Industry Terms:
  • Not Available to Industry