OTR untagged
(Plasmid
#208913)
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Purposeencodes the human oxytocin receptor, without any tags
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 208913 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1 or 3.0
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5400
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameOXTR
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SpeciesH. sapiens (human)
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Insert Size (bp)1200
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MutationTM5: 5x56, A218, Threonin instead of Alanin
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Entrez GeneOXTR (a.k.a. OT-R, OTR)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SacI (unknown if destroyed)
- 3′ cloning site BamHI (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
OTR untagged was a gift from Christian Gruber (Addgene plasmid # 208913 ; http://n2t.net/addgene:208913 ; RRID:Addgene_208913) -
For your References section:
Nature-inspired dimerization as a strategy to modulate neuropeptide pharmacology exemplified with vasopressin and oxytocin. Dekan Z, Kremsmayr T, Keov P, Godin M, Teakle N, Durrauer L, Xiang H, Gharib D, Bergmayr C, Hellinger R, Gay M, Vilaseca M, Kurzbach D, Albericio F, Alewood PF, Gruber CW, Muttenthaler M. Chem Sci. 2021 Feb 4;12(11):4057-4062. doi: 10.1039/d0sc05501h. 10.1039/d0sc05501h PubMed 34163676