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Addgene

pCAG2LMKOSimO
(Plasmid #20866)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20866 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAG
  • Backbone size w/o insert (bp) 6397
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5a
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    c-Myc-F2A-Klf4-T2A-Oct4-E2A-Sox2-ires-mOrange
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    6302
  • GenBank ID
    BC006728 NM_011443 NM_013633 NM_010637
  • Entrez Gene
    Myc (a.k.a. Myc2, Niard, Nird, bHLHe39)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG2LMKOSimO was a gift from Keisuke Kaji (Addgene plasmid # 20866 ; http://n2t.net/addgene:20866 ; RRID:Addgene_20866)

  • For your References section:

    Virus-free induction of pluripotency and subsequent excision of reprogramming factors. Kaji K, Norrby K, Paca A, Mileikovsky M, Mohseni P, Woltjen K. Nature. 2009 Mar 1. ():. 10.1038/nature07864 PubMed 19252477
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