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Addgene

pCAGMKOSiE
(Plasmid #20865)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20865 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAG-ires-EGFP
  • Backbone size w/o insert (bp) 6397
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5a
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    myelocytomatosis oncogene-F2A-Kruppel-like factor 4-T2A-POU domain, class 5, transcription factor 1-E2A-SRY-box containing gene
  • Alt name
    c-Myc-F2A-Klf4-T2A-Oct4-E2A-Sox2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    4971
  • GenBank ID
    BC006728 NM_011443 NM_013633 NM_010637
  • Entrez Gene
    Myc (a.k.a. Myc2, Niard, Nird, bHLHe39)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAGMKOSiE was a gift from Keisuke Kaji (Addgene plasmid # 20865 ; http://n2t.net/addgene:20865 ; RRID:Addgene_20865)

  • For your References section:

    Virus-free induction of pluripotency and subsequent excision of reprogramming factors. Kaji K, Norrby K, Paca A, Mileikovsky M, Mohseni P, Woltjen K. Nature. 2009 Mar 1. ():. 10.1038/nature07864 PubMed 19252477
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