SuperTopAP
(Plasmid #20334)

• Depositing Lab: Connie Cepko
• Publication: Cho et al Development. 2006 Aug . 133(16):3167-77.

Backbone

• Vector backbone: M50
• Backbone size w/o insert (bp): 4800
• Vector type: AP reporter

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: beta catenin reporter
• Species: H. sapiens (human)
• Insert Size (bp): 2200
• Entrez Gene: CTNNB1 (a.k.a. CTNNB, EVR7, MRD19, NEDSDV, armadillo)
• Tag / Fusion Protein:
  • AP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (unknown if destroyed)
• 3′ cloning site: HpaI (unknown if destroyed)
• 5′ sequencing primer: RVprimer3

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The idea for the construct is from Hans Clevers lab, who designed the original TOPflash.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry