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Addgene

pUC19/PLIN2-GFP
(Plasmid #202194)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 202194 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC19
  • Backbone size w/o insert (bp) 2686
  • Total vector size (bp) 4600
  • Modifications to backbone
    added C-terminal PLIN2 homology arms
  • Vector type
    cloning vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PLIN2
  • Alt name
    GFP knock-in to C-terminus of PLIN2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1897
  • GenBank ID
    NM_001122.4
  • Entrez Gene
    PLIN2 (a.k.a. ADFP, ADRP)
  • Promoter lac
  • Tag / Fusion Protein
    • GFP knock-in

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer M13/pUC Reverse
  • 3′ sequencing primer M13 (-21) Forward
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC19/PLIN2-GFP was a gift from James Olzmann (Addgene plasmid # 202194 ; http://n2t.net/addgene:202194 ; RRID:Addgene_202194)

  • For your References section:

    Parallel CRISPR-Cas9 screens identify mechanisms of PLIN2 and lipid droplet regulation. Roberts MA, Deol KK, Mathiowetz AJ, Lange M, Leto DE, Stevenson J, Hashemi SH, Morgens DW, Easter E, Heydari K, Nalls MA, Bassik MC, Kampmann M, Kopito RR, Faghri F, Olzmann JA. Dev Cell. 2023 Jul 18:S1534-5807(23)00331-3. doi: 10.1016/j.devcel.2023.07.001. 10.1016/j.devcel.2023.07.001 PubMed 37494933
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