pX330 PLIN2
(Plasmid #202196)

• Purpose: pX330 backbone expressing sgRNA to edit the C-terminus of human PLIN2
• Depositing Lab: James Olzmann
• Publication: Roberts et al Dev Cell. 2023 Jul 18:S1534-5807(23)00331-3. doi: 10.1016/j.devcel.2023.07.001.

Backbone

• Vector backbone: pX330
• Backbone manufacturer: Feng Zhang lab (Addgene plasmid # 42230)
• Backbone size w/o insert (bp): 8484
• Total vector size (bp): 8503
• Vector type: Mammalian Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PLIN2
• Species: H. sapiens (human)
• Insert Size (bp): 19
• GenBank ID: NM_001122.4
• Entrez Gene: PLIN2 (a.k.a. ADFP, ADRP)
• Promoter: U6

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BbsI (destroyed during cloning)
• 3′ cloning site: BbsI (destroyed during cloning)
• 5′ sequencing primer: LKO.1 5'

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry