pYS28
(Plasmid #202085)

• Purpose: Parent vector for producing universal minicircle donors for HaloTag C-terminal knock-in
• Depositing Lab: Daniel Dickinson
• Publication: Shi et al Development. 2023 May 15;150(10):dev201367. doi: 10.1242/dev.201367. Epub 2023 May 25.

Backbone

• Vector backbone: MN100A-1
• Backbone manufacturer: System Biosciences
• Vector type: CRISPR
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CT Halo-T2A-HygR Minicircle Parent
• Species: Synthetic
• Promoter: None
• Tag / Fusion Protein:
  • HaloTag (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning

Resource Information

• Supplemental Documents:
  • pYS28.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • HaloTag Vector Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

IMPORTANT: This plasmid is shipped from Addgene in NEB Stable cells, but these cells are NOT suitable for minicircle production. You will need to purchase ZYCY10P3S2T cells separately, and transform the plasmid into that host strain, to produce minicircles.