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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 19765 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneFUdeltaGW
- Backbone size w/o insert (bp) 9941
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Growth instructionsStbl3
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSox2
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SpeciesM. musculus (mouse)
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Insert Size (bp)950
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Entrez GeneSox2 (a.k.a. Sox-2, lcc, ysb)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
From article: A tet-inducible lentivirus called LV-tetO was generated by removing the EcoRI/PacI fragment containing the ubiquitin promoter elements from the FUdeltaGW vector (modified from FUGW by removal of GFP with EcoRI-XbaI and re-creation of the EcoRI site) and replacing it with tetO sequences derived from pTet-Splice by EcoRI/XhoI digestion.cDNAs for c-Myc (T58A mutant), Klf4, Oct4, and Sox2 were isolated from pMIG or pMX vectors and cloned into LV-tetO using a unique EcoRI restriction site.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLV-tetO-Sox2 was a gift from Konrad Hochedlinger (Addgene plasmid # 19765 ; http://n2t.net/addgene:19765 ; RRID:Addgene_19765) -
For your References section:
Defining molecular cornerstones during fibroblast to iPS cell reprogramming in mouse. Stadtfeld M, Maherali N, Breault DT, Hochedlinger K. Cell Stem Cell. 2008 Mar 6. 2(3):230-40. 10.1016/j.stem.2008.02.001 PubMed 18371448