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pGEX-4T-1-β4 hinge-ear
(Plasmid #196692)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 196692 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-4T-1
  • Backbone size w/o insert (bp) 4969
  • Total vector size (bp) 5604
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    AP-4 β4 hinge-ear (534-739)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    639
  • Entrez Gene
    AP4B1 (a.k.a. BETA-4, CPSQ5, SPG47)
  • Promoter tac
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SmaI (destroyed during cloning)
  • 3′ cloning site SmaI (destroyed during cloning)
  • 5′ sequencing primer GST forward
  • 3′ sequencing primer GST reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Contains 15 extra nucleotides encoding sequence MAMEA at 5' of insert

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-4T-1-β4 hinge-ear was a gift from Juan Bonifacino (Addgene plasmid # 196692 ; http://n2t.net/addgene:196692 ; RRID:Addgene_196692)

  • For your References section:

    Bivalent Motif-Ear Interactions Mediate the Association of the Accessory Protein Tepsin with the AP-4 Adaptor Complex. Mattera R, Guardia CM, Sidhu SS, Bonifacino JS. J Biol Chem. 2015 Dec 25;290(52):30736-49. doi: 10.1074/jbc.M115.683409. Epub 2015 Nov 5. 10.1074/jbc.M115.683409 PubMed 26542808
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