FKBP-beta3(702-1094)-GFP
(Plasmid
#100734)
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PurposeTo express a clathrin "hook" in mammalian cells to hot-wire endocytosis
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 100734 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-N1
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAP3B1
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Alt namebeta3 adaptin
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SpeciesH. sapiens (human)
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Mutationappendage and ear only
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GenBank IDNM_001271769.1
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Entrez GeneAP3B1 (a.k.a. ADTB3, ADTB3A, HPS, HPS2, PE)
- Promoter CMV
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Tags
/ Fusion Proteins
- FKBP (N terminal on insert)
- GFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site AgeI (unknown if destroyed)
- 3′ cloning site PspOMI (unknown if destroyed)
- 5′ sequencing primer CMV Forward (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
FKBP-beta3(702-1094)-GFP was a gift from Stephen Royle (Addgene plasmid # 100734 ; http://n2t.net/addgene:100734 ; RRID:Addgene_100734) -
For your References section:
New tools for "hot-wiring" clathrin-mediated endocytosis with temporal and spatial precision. Wood LA, Larocque G, Clarke NI, Sarkar S, Royle SJ. J Cell Biol. 2017 Sep 27. pii: jcb.201702188. doi: 10.1083/jcb.201702188. 10.1083/jcb.201702188 PubMed 28954824