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Addgene

pGTVL2-CRBN-TBD
(Plasmid #196369)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 196369 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pGTVL2
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CRBN
  • Species
    H. sapiens (human)
  • Mutation
    contains only T319-T425
  • Entrez Gene
    CRBN (a.k.a. MRT2, MRT2A)
  • Promoter T7
  • Tag / Fusion Protein
    • His6-GST-TEV (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGTVL2-CRBN-TBD was a gift from Stefan Knapp (Addgene plasmid # 196369 ; http://n2t.net/addgene:196369 ; RRID:Addgene_196369)