pGTVL2-CRBN-TBD
(Plasmid
#196369)
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Purposeprotein expression of the GST-tagged Cereblon Thalidomide binding domain
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 196369 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGTVL2
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCRBN
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SpeciesH. sapiens (human)
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Mutationcontains only T319-T425
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Entrez GeneCRBN (a.k.a. MRT2, MRT2A)
- Promoter T7
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Tag
/ Fusion Protein
- His6-GST-TEV (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGTVL2-CRBN-TBD was a gift from Stefan Knapp (Addgene plasmid # 196369 ; http://n2t.net/addgene:196369 ; RRID:Addgene_196369)