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pET-11b_PcrA_M6_H93A
(Plasmid #194156)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 194156 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-11b
  • Backbone manufacturer
    N/A
  • Backbone size w/o insert (bp) 5676
  • Total vector size (bp) 7971
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Protein expression in BL21 (DE3) pLysS
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Engineered helicase PcrA_M6
  • Species
    N/A
  • Insert Size (bp)
    2295
  • Mutation
    H93A, C96A, N187C, C247A, L384V, L409C
  • Tags / Fusion Proteins
    • 6x His tag (N terminal on insert)
    • FLAG tag (C terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-11b_PcrA_M6_H93A was a gift from Taekjip Ha (Addgene plasmid # 194156 ; http://n2t.net/addgene:194156 ; RRID:Addgene_194156)

  • For your References section:

    Engineered helicase replaces thermocycler in DNA amplification while retaining desired PCR characteristics. Gavrilov M, Yang JYC, Zou RS, Ma W, Lee CY, Mohapatra S, Kang J, Liao TW, Myong S, Ha T. Nat Commun. 2022 Oct 23;13(1):6312. doi: 10.1038/s41467-022-34076-0. 10.1038/s41467-022-34076-0 PubMed 36274095
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