Skip to main content
Addgene

pCFJ104 - Pmyo-3::mCherry::unc-54
(Plasmid #19328)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 19328 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pDESTR4-R3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2416
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Pmyo-3::mCherry
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    4224
  • Tag / Fusion Protein
    • mCherry

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site attR4 (destroyed during cloning)
  • 3′ cloning site attR3 (destroyed during cloning)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Red mCherry expression in the pharynx driven by the myo-3 promoter. unc-54 utr.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCFJ104 - Pmyo-3::mCherry::unc-54 was a gift from Erik Jorgensen (Addgene plasmid # 19328 ; http://n2t.net/addgene:19328 ; RRID:Addgene_19328)

  • For your References section:

    Single-copy insertion of transgenes in Caenorhabditis elegans. Christian Frøkjær-Jensen, M Wayne Davis, Christopher E Hopkins, Blake J Newman, Jason M Thumme, Søren-Peter Olesen, Morten Grunnet & Erik M Jorgensen. 10.1038/ng.248 PubMed 18953339
Included in kit:
Related items:
Commonly requested with: