iCAP-ROOT
(Plasmid
#192262)
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PurposeAAV9 derived Retrograde vector Optimized for Organ Tracing (ROOT) capsid
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 192262 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC57-mini
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSynthetic construct for ROOT VP1 gene
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Alt nameROOT
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SpeciesSynthetic
- Promoter p41
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that the rep-cap construct is generated based on tTA-TRE amplification vector from Gradinaru lab (Addgene #103006; PubMed 28671695).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
iCAP-ROOT was a gift from Li Ye (Addgene plasmid # 192262 ; http://n2t.net/addgene:192262 ; RRID:Addgene_192262) -
For your References section:
The role of somatosensory innervation of adipose tissues. Wang Y, Leung VH, Zhang Y, Nudell VS, Loud M, Servin-Vences MR, Yang D, Wang K, Moya-Garzon MD, Li VL, Long JZ, Patapoutian A, Ye L. Nature. 2022 Sep;609(7927):569-574. doi: 10.1038/s41586-022-05137-7. Epub 2022 Aug 31. 10.1038/s41586-022-05137-7 PubMed 36045288