pLenti-eGFP-dSaCas9-blast
(Plasmid #191653)

• Purpose: Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9), eGFP, and the blasticidin resistance gene.
• Depositing Lab: Moshe Szyf
• Publication: Sapozhnikov et al Sci Rep. 2023 Feb 9;13(1):2280. doi: 10.1038/s41598-023-29332-2.

Backbone

• Vector backbone: pLenti- V6.3 Ultra
• Backbone manufacturer: Addgene #106172
• Vector type: Mammalian Expression, Lentiviral, CRISPR
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: eGFP-T2A-dSaCas9
• Alt name: SadCas9
• Alt name: dSaCas9
• Species: Synthetic
• Promoter: CMV
• Tag / Fusion Protein:
  • 3XFLAG (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CMV-F

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Sa-dCas9-NLS-3xFLAG/pcDNA3.1 (Addgene plasmid #98041)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry