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Addgene

pABCb10-GFP
(Plasmid #19031)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 19031 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIRES
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 6092
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ATP-binding cassette, sub-family B (MDR/TAP), member 10
  • Alt name
    ABCme
  • Alt name
    ABCb10
  • Alt name
    Abc-mitochondrial erythroid
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2147
  • GenBank ID
    NM_019552
  • Entrez Gene
    Abcb10 (a.k.a. AU016331, Abc-me, Abcb12, C76534)
  • Tag / Fusion Protein
    • HIS (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site MluI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer IRES-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pABCb10-GFP was a gift from Orian Shirihai (Addgene plasmid # 19031 ; http://n2t.net/addgene:19031 ; RRID:Addgene_19031)

  • For your References section:

    Targeting, import, and dimerization of a mammalian mitochondrial ATP binding cassette (ABC) transporter, ABCB10 (ABC-me). Graf SA, Haigh SE, Corson ED, Shirihai OS. J Biol Chem. 2004 Oct 8. 279(41):42954-63. 10.1074/jbc.M405040200 PubMed 15215243
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