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Addgene

FKBP-AP180-GFP
(Plasmid #186577)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 186577 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone manufacturer
    Clontech
  • Total vector size (bp) 7369
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AP180
  • Alt name
    SNAP91
  • Species
    R. norvegicus (rat)
  • Entrez Gene
    Snap91 (a.k.a. Ap180)
  • Tag / Fusion Protein
    • FKBP N-terminal GFP C-terminal

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site AgeI (not destroyed)
  • 5′ sequencing primer pCMV
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note: The plasmid is difficult to sequence near bases 2140-2220 that are within the insert.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FKBP-AP180-GFP was a gift from Stephen Royle (Addgene plasmid # 186577 ; http://n2t.net/addgene:186577 ; RRID:Addgene_186577)

  • For your References section:

    Recruitment of clathrin to intracellular membranes is sufficient for vesicle formation. Kuey C, Sittewelle M, Larocque G, Hernandez-Gonzalez M, Royle SJ. Elife. 2022 Jul 19;11. pii: 78929. doi: 10.7554/eLife.78929. 10.7554/eLife.78929 PubMed 35852853
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