USO hisB- pyrF- rpoZ-
(Bacterial strain #18049)

• Depositing Lab: Scot Wolfe
• Publication: Noyes et al Nucleic Acids Res. 2008 Mar 10. ():.

Backbone

• Vector backbone: XL-1 blue F' episome
• Vector type: Bacterial Expression
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Tetracycline, 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: omega knockout Bacteria hybrid selection strain
• Insert Size (bp): 450
• Mutation: This is the US0 selection strain that has hisB, pyrF and rpoZ knocked out. A zeocin cassette has been knocked in to the rpoZ locus

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: N/A

Resource Information

• Articles Citing this Plasmid:
  • 9 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This strain has a deletion in hisB, pyrF and ropZ genes (the bacterial homologs of HIS3 and URA3 and the omega subunit) and it contains a F' episome bearing the lacl^q repressor.