-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 18040 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backboneColE1
- Backbone size w/o insert (bp) 3605
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namePaired
-
SpeciesD. melanogaster (fly)
-
Insert Size (bp)800
-
MutationPaired is simply used as an insert between Kpn1 and Xba1 to be dropped out for cloning.
-
Entrez Geneprd (a.k.a. Dmel_CG6716, CG6716, Dmel\CG6716, PRD, Prd, pr)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Kpn1 (not destroyed)
- 3′ cloning site Xba1 (not destroyed)
- 5′ sequencing primer OMG5 (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pB1H2wL-Prd was a gift from Scot Wolfe (Addgene plasmid # 18040 ; http://n2t.net/addgene:18040 ; RRID:Addgene_18040) -
For your References section:
A systematic characterization of factors that regulate Drosophila segmentation via a bacterial one-hybrid system. Noyes MB, Meng X, Wakabayashi A, Sinha S, Brodsky MH, Wolfe SA. Nucleic Acids Res. 2008 Mar 10. ():. 10.1093/nar/gkn048 PubMed 18332042