pGL3-NFAT luciferase
(Plasmid #17870)

• Purpose: 3x NFAT binding sequence upstream of a luciferase reporter; can be used to assay signal transduction through Ca2+, calcineurin and NFAT in any tissue
• Depositing Lab: Jerry Crabtree
• Publication: Clipstone et al Nature. 1992 Jun 25. 357(6380):695-7.

Backbone

• Vector backbone: pGL3
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 4800
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: 3x NFAT binding sequence
• Alt name: NF-AT site
• Entrez Gene: IL2 (a.k.a. IL-2, TCGF, lymphokine)
• Promoter: IL-2 promoter
• Tags / Fusion Proteins:
  • IL2 promoter (C terminal on insert)
  • Luciferase (C terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: RVprimer3 (5'-CTAGCAAAATAGGCTGTCCC-3')
• 3′ sequencing primer: LucNrev

Resource Information

• Articles Citing this Plasmid:
  • 36 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid has three copies of the NF-AT site cloned upstream of the minimal IL-2 promoter from -89 to +51.

The plasmid can be used to assay signal transduction through Ca2+, calcineurin and NFAT in any tissue including lymphocytes, neurons, osteoblasts, skin, endothelium, skeletal and heart muscle (Shaw et al Science 1988; Crabtree and Schreiber Cell 138,210, 2009).

Based on Addgene's full plasmid sequence, the NFAT binding sequence appears to be ACGCCTTCTGTATGAAACAGTTTTTCCTCC.