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pLX304-DCK*-GW-IRES-GFP
(Plasmid #176289)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 176289 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLX304
  • Backbone manufacturer
    None
  • Backbone size w/o insert (bp) 11000
  • Total vector size (bp) 12000
  • Modifications to backbone
    an IRES-GFP cassette was sub cloned into the NheI-AgeI site. To maintain frame between the DCK* suicide gene, and the gene of interest cloned into the Gateway cassette, please make sure to be in-frame with the TACAAAAAA in the attR1 site, and the TACAAAAAA in the attR2 site.
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    ccdB Survival
  • Growth instructions
    Longer growth may be needed
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Mutant Deoxycytidine Kinase (DCK*, S74E R104M D133A)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    778
  • Mutation
    S74E R104M D133A
  • GenBank ID
    NM_000788.3
  • Entrez Gene
    DCK
  • Tag / Fusion Protein
    • V5 (C terminal on backbone)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Mutant deoxycytidine kinase was the kind gift of the Medin Lab

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLX304-DCK*-GW-IRES-GFP was a gift from William Kaelin (Addgene plasmid # 176289 ; http://n2t.net/addgene:176289 ; RRID:Addgene_176289)

  • For your References section:

    Targeting oncoproteins with a positive selection assay for protein degraders. Koduri V, Duplaquet L, Lampson BL, Wang AC, Sabet AH, Ishoey M, Paulk J, Teng M, Harris IS, Endress JE, Liu X, Dasilva E, Paulo JA, Briggs KJ, Doench JG, Ott CJ, Zhang T, Donovan KA, Fischer ES, Gygi SP, Gray NS, Bradner J, Medin JA, Buhrlage SJ, Oser MG, Kaelin WG Jr. Sci Adv. 2021 Feb 5;7(6). pii: 7/6/eabd6263. doi: 10.1126/sciadv.abd6263. Print 2021 Feb. 10.1126/sciadv.abd6263 PubMed 33547076
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