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Addgene

-204 human IFN-gamma luc
(Plasmid #17596)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17596 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL3-basic
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4800
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    IFN gamma promoter
  • Alt name
    interferon gamma
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    240
  • Mutation
    -204 to +38 of promoter
  • Entrez Gene
    IFNG (a.k.a. IFG, IFI, IMD69)
  • Tag / Fusion Protein
    • luciferase (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BstXI (not destroyed)
  • 3′ cloning site BstXI (not destroyed)
  • 5′ sequencing primer RVprimer3
  • 3′ sequencing primer LucNrev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    -204 human IFN-gamma luc was a gift from Howard Young (Addgene plasmid # 17596 ; http://n2t.net/addgene:17596 ; RRID:Addgene_17596)

  • For your References section:

    Mucosa-specific targets for regulation of IFN-gamma expression: lamina propria T cells use different cis-elements than peripheral blood T cells to regulate transactivation of IFN-gamma expression. Gonsky R, Deem RL, Bream JH, Lee DH, Young HA, Targan SR. J Immunol. 2000 Feb 1. 164(3):1399-407. PubMed 10640755
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