AAV2_HLF-ZP
(Plasmid
#175035)
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PurposeHLF targeting construct introducing an ires ZsG P2A Puro cassette downstream of the human HLF stop codon
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 175035 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV2
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Vector typeAAV
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Selectable markerstEGFR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHLF ires ZsGreen P2A Puro
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SpeciesH. sapiens (human)
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Insert Size (bp)3857
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GenBank IDNM_002126
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AAV2_HLF-ZP was a gift from Guy Sauvageau (Addgene plasmid # 175035 ; http://n2t.net/addgene:175035 ; RRID:Addgene_175035) -
For your References section:
HLF Expression Defines the Human Hematopoietic Stem Cell State. Lehnertz B, Chagraoui J, MacRae T, Tomellini E, Corneau S, Mayotte N, Boivin I, Durand A, Gracias D, Sauvageau G. Blood. 2021 Sep 9. pii: S0006-4971(21)01571-8. doi: 10.1182/blood.2021010745. 10.1182/blood.2021010745 PubMed 34499717