pNCA
(Plasmid #17363)

• Depositing Lab: Stephen Goff
• Publication: Colicelli et al J Mol Biol. 1988 Jan 5. 199(1):47-59.

Backbone

• Vector backbone: pBR322
• Backbone size w/o insert (bp): 2100

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: MMLV proviral DNA
• Species: Moloney murine leukemia virus
• Insert Size (bp): 8000

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: ClaI (unknown if destroyed)
• 3′ cloning site: NdeI (unknown if destroyed)
• 5′ sequencing primer: n/a

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The plasmid pA8.2 is a full-length permuted copy of
the M-MuLV genome in the vector pACYC177 (Lobel &
Goff, 1984). The full-length, non-permuted M-MuLV
clone pNCA was constructed from the 5.5 kb ClaI-
HindIII fragment of pA8.2, the 2.5 kb HindIII-NdeI
fragment of pNDE (Colicelli & Goff. 1985) and the 2.1 kb
ClaI-NdeI fragment of pBR322. It is, in effect, an 11kb
non-permuted wild-type clone reconstructed from a
permuted clone and with no flanking cellular sequences.