pGST-PPARdelta
(Plasmid #16548)

• Depositing Lab: Bert Vogelstein
• Publication: He et al Cell. 1999 Oct 29. 99(3):335-45.

Backbone

• Vector backbone: pGEX-2TK
• Backbone manufacturer: Amersham
• Backbone size w/o insert (bp): 5000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PPAR delta
• Species: H. sapiens (human)
• Insert Size (bp): 747
• Mutation: N-terminal DNA-binding domain: aa 1-249
• Entrez Gene: PPARD (a.k.a. FAAR, NR1C2, NUC1, NUCI, NUCII, PPARB)
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: pGEX5'
• 3′ sequencing primer: pGEX3'

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry