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Addgene

pGST-RXRalpha
(Plasmid #16547)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16547 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pGEX-2TK
  • Backbone manufacturer
    Amersham
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RXR alpha
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    672
  • Mutation
    N-terminal DNA-binding domain: aa 1-224
  • Entrez Gene
    RXRA (a.k.a. NR2B1, RXR-alpha, RXRalpha)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pGEX5'
  • 3′ sequencing primer pGEX3'
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note that there are some discrepancies between the insert in this plasmid and the canonical GenBank PPARgamma. The Vogelstein lab maintains that this plasmid worked as specified when they made and used it.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGST-RXRalpha was a gift from Bert Vogelstein (Addgene plasmid # 16547 ; http://n2t.net/addgene:16547 ; RRID:Addgene_16547)
  • For your References section:

    PPARdelta is an APC-regulated target of nonsteroidal anti-inflammatory drugs. He TC, Chan TA, Vogelstein B, Kinzler KW. Cell. 1999 Oct 29. 99(3):335-45. 10.1016/S0092-8674(00)81664-5 PubMed 10555149