Axin GID 5-6 LP/pCS2MT
(Plasmid
#16302)
-
Depositing Lab
-
Publication
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 16302 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepCS2+MT
- Backbone size w/o insert (bp) 4352
-
Vector typeVertebrate expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameAxin GID 5-6 LP
-
Alt nameAxin
-
SpeciesX. laevis (frog)
-
Insert Size (bp)78
-
MutationAmino acids 380-404, L to P mutation
-
Entrez Geneaxin1.L (a.k.a. XELAEV_18045581mg, axin1, fused)
-
Tag
/ Fusion Protein
- 5x myc (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
GID=Axin GSK3 interaction domain. L->P is an inactive control. It does not bind GSK-3 and does not inhibit GSK-3. For sense transcripts, cut with NotI.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
Axin GID 5-6 LP/pCS2MT was a gift from Peter Klein (Addgene plasmid # 16302 ; http://n2t.net/addgene:16302 ; RRID:Addgene_16302) -
For your References section:
Regulation of glycogen synthase kinase 3beta and downstream Wnt signaling by axin. Hedgepeth CM, Deardorff MA, Rankin K, Klein PS. Mol Cell Biol. 1999 Oct . 19(10):7147-57. PubMed 10490650